Abstract

The rapid growth of the cannabis market in the United States has led to increasing focus on the consumer product safety of the myriad of new products entering commerce. The cannabis plant is widely accepted to be an efficient accumulator of potentially toxic elements, and its resinous nature makes it well suited to accumulate surface contaminants. Unfortunately, limited data are currently available on the occurrence of many elements in consumer cannabis, and even fewer studies have examined surface adhered particulate matter. As part of this study, 26 elements (silver, aluminum, arsenic, barium, beryllium, calcium, cadmium, cobalt, chromium, copper, iron, mercury, potassium, magnesium, manganese, molybdenum, sodium, nickel, lead, antimony, selenium, thorium, thallium, uranium, vanadium, and zinc) were quantified in hemp produced for cannabidiol and commercially available hemp marketed for smoking. Additionally, surface adhered particulate matter was examined as a potential contributor of elemental impurities, and various methodologies were compared. Results confirm that hemp entering commerce in the United States contains a variety of elemental impurities and frequently contains adhered particulate matter such as soil minerals, agricultural additives, microplastics/textile fibers, and materials from harvesting/processing equipment. Consumer exposure potential for some elements such as copper was found to be high enough to warrant additional investigation as to the possible health effects and may justify additional oversight from regulators, who should consider expanding testing panels to a larger suite of elements than arsenic, cesium, mercury, and lead.

References

1.
Hurd
Y. L.
, “
Leading the Next CBD Wave-Safety and Efficacy
,”
JAMA Psychiatry
77
, no. 
4
(April
2020
):
341
342
, https://doi.org/10.1001/jamapsychiatry.2019.4157
2.
Chopra
A. S.
,
Lordan
R.
,
Horbańczuk
O. K.
,
Atanasov
A. G.
,
Chopra
I.
,
Horbańczuk
J. O.
,
Jóźwik
A.
, et al., “
The Current Use and Evolving Landscape of Nutraceuticals
,”
Pharmacological Research
175
(
2022
): 106001, https://doi.org/10.1016/j.phrs.2021.106001
3.
Schauer
G. L.
,
Johnson
J. K.
,
Rak
D. J.
,
Dodson
L.
,
Steinfeld
N.
,
Sheehy
T. J.
,
Nakata
M.
, and
Collins
S. P.
, “
A Research Agenda to Inform Cannabis Regulation: How Science Can Shape Policy
,”
Clinical Therapeutics
45
, no. 
6
(June
2023
):
506
514
, https://doi.org/10.1016/j.clinthera.2023.03.010
4.
Bengyella
L.
,
Kuddus
M.
,
Mukherjee
P.
,
Fonmboh
D. J.
, and
Kaminski
J. E.
, “
Global Impact of Trace Non-essential Heavy Metal Contaminants in Industrial Cannabis Bioeconomy
,”
Toxin Reviews
41
, no. 
4
(
2022
):
1215
1225
, https://doi.org/10.1080/15569543.2021.1992444
5.
Wu
Y.
,
Trejo
H. X.
,
Chen
G.
, and
Li
S.
, “
Phytoremediation of Contaminants of Emerging Concern from Soil with Industrial Hemp (Cannabis sativa L.): A Review
,”
Environment, Development and Sustainability
23
, no. 
10
(October
2021
):
14405
14435
, https://doi.org/10.1007/s10668-021-01289-0
6.
Sikorski
C.
,
Leos-Toro
C.
, and
Hammond
D.
, “
Cannabis Consumption, Purchasing and Sources among Young Canadians: The Cannabis Purchase and Consumption Tool (CPCT)
,”
Substance Use and Misuse
56
, no. 
4
(
2021
):
449
457
, https://doi.org/10.1080/10826084.2021.1879142
7.
Balali-Mood
M.
,
Naseri
K.
,
Tahergorabi
Z.
,
Khazdair
M. R.
, and
Sadeghi
M.
, “
Toxic Mechanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium, and Arsenic
,”
Frontiers in Pharmacology
12
(
2021
): 643972, https://doi.org/10.3389/fphar.2021.643972
8.
McPartland
J. M.
and
McKernan
K. J.
, “
Contaminants of Concern in Cannabis: Microbes, Heavy Metals and Pesticides
,” in
Cannabis sativa L. - Botany and Biotechnology
, eds.
Chandra
S.
,
Lata
H.
, and
El Sohly
M. A.
(
Cham, Switzerland
:
Springer
,
2017
),
457
474
, https://doi.org/10.1007/978-3-319-54564-6_22
9.
Deng
Y.
,
Wang
M.
,
Tian
T.
,
Lin
S.
,
Xu
P.
,
Zhou
L.
,
Dai
C.
, et al., “
The Effect of Hexavalent Chromium on the Incidence and Mortality of Human Cancers: A Meta-Analysis Based on Published Epidemiological Cohort Studies
,”
Frontiers in Oncology
9
(
2019
): 24, https://doi.org/10.3389/fonc.2019.00024
10.
Gosens
I.
,
Cassee
F. R.
,
Zanella
M.
,
Manodori
L.
,
Brunelli
A.
,
Costa
A. L.
,
Bokkers
B. G. H.
, et al., “
Organ Burden and Pulmonary Toxicity of Nano-sized Copper (II) Oxide Particles after Short-Term Inhalation Exposure
,”
Nanotoxicology
10
, no. 
8
(September
2016
):
1084
1095
, https://doi.org/10.3109/17435390.2016.1172678
11.
Costa
P. M.
,
Gosens
I.
,
Williams
A.
,
Farcal
L.
,
Pantano
D.
,
Brown
D. M.
,
Stone
V.
,
Cassee
F. R.
,
Halappanavar
S.
, and
Fadeel
B.
, “
Transcriptional Profiling Reveals Gene Expression Changes Associated with Inflammation and Cell Proliferation Following Short-Term Inhalation Exposure to Copper Oxide Nanoparticles
,”
Journal of Applied Toxicology
38
, no. 
3
(March
2018
):
385
397
, https://doi.org/10.1002/jat.3548
12.
Moir
D.
,
Rickert
W. S.
,
Levasseur
G.
,
Larose
Y.
,
Maertens
R.
,
White
P.
, and
Desjardins
S.
, “
A Comparison of Mainstream and Sidestream Marijuana and Tobacco Cigarette Smoke Produced under Two Machine Smoking Conditions
,”
Chemical Research in Toxicology
21
, no. 
2
(February
2008
):
494
502
, https://doi.org/10.1021/tx700275p
13.
Stavrides
J. C.
, “
Lung Carcinogenesis: Pivotal Role of Metals in Tobacco Smoke
,”
Free Radical Biology and Medicine
41
, no. 
7
(October
2006
):
1017
1030
, https://doi.org/10.1016/j.freeradbiomed.2006.06.024
14.
United States Environmental Protection Agency “
Method 6020B (SW-846): Inductively Coupled Plasma - Mass Spectrometry
,”
Revision 2
(
Washington, DC
:
United States Environmental Protection Agency
,
2014
).
15.
Standard Test Method for Analysis of Multiple Elements in Cannabis Matrices by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
, ASTM D8469−22 (West Conshohocken, PA:
ASTM International
, approved August 1,
2022
), https://doi.org/10.1520/D8469-22
16.
Zumbado
M.
,
Luzardo
O. P.
,
Rodríguez-Hernández
Á.
,
Boada
L. D.
, and
Henríquez-Hernández
L. A.
, “
Differential Exposure to 33 Toxic Elements through Cigarette Smoking, Based on the Type of Tobacco and Rolling Paper Used
,”
Environmental Research
169
(
2019
):
368
376
, https://doi.org/10.1016/j.envres.2018.11.021
17.
Caulkins
J. P.
,
Pardo
B.
, and
Kilmer
B.
, “
Intensity of Cannabis Use: Findings from Three Online Surveys
,”
International Journal on Drug Policy
79
(
2020
): 102740, https://doi.org/10.1016/j.drugpo.2020.102740
18.
Douvris
C.
,
Bentil
E.
,
Ayensu
I.
,
Osei Akoto
C.
,
Amponsah
I. K.
,
Adu
J.
, and
Bussan
D.
, “
Trace Metals in Cannabis Seized by Law Enforcement in Ghana and Multivariate Analysis to Distinguish among Different Cannabis Farms
,”
Toxics
10
, no. 
10
(October
2022
): 567, https://doi.org/10.3390/toxics10100567
19.
Collin
S.
,
Baskar
A.
,
Geevarghese
D. M.
,
Ali
M. N. V. S.
,
Bahubali
P.
,
Choudhary
R.
,
Lvov
V.
, et al., “
Bioaccumulation of Lead (Pb) and Its Effects in Plants: A Review
,”
Journal of Hazardous Materials Letters
3
(
2022
): 100064, https://doi.org/10.1016/j.hazl.2022.100064
20.
United States Pharmacopeia “
(232) Elemental Impurities—Limits
,” in
Chemical Tests 1
(Rockville, MD: United States Pharmacopeia,
2023
), http://web.archive.org/web/20240528162619/https:/www.usp.org/sites/default/files/usp/document/our-work/chemical-medicines/key-issues/c232-usp-39.pdf
21.
International Council for Harmonisation “
Guideline for Elemental Impurities Q3D(R1)
,” in
International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use
(
Amsterdam, the Netherlands
:
European Medicines Agency
,
2019
).
22.
Thomas
R. J.
,
Measuring Heavy Metal Contaminants in Cannabis and Hemp
(
Boca Raton, FL
:
CRC Press
,
2021
).
23.
Mielke
H. W.
,
Gonzales
C. R.
,
Powell
E. T.
, and
Egendorf
S. P.
, “
Lead in Air, Soil, and Blood: Pb Poisoning in a Changing World
,”
International Journal of Environmental Research and Public Health
19
, no. 
15
(August
2022
): 9500, https://doi.org/10.3390/ijerph19159500
24.
Frank
J. J.
,
Poulakos
A. G.
,
Tornero-Velez
R.
, and
Xue
J.
, “
Systematic Review and Meta-Analyses of Lead (Pb) Concentrations in Environmental Media (Soil, Dust, Water, Food, and Air) Reported in the United States from 1996 to 2016
,”
Science of the Total Environment
694
(
2019
): 133489, https://doi.org/10.1016/j.scitotenv.2019.07.295
25.
World Health Organization,
WHO Global Air Quality Guidelines. Particulate Matter (PM2.5 and PM10), Ozone, Nitrogen Dioxide, Sulfur Dioxide and Carbon Monoxide
(Geneva, Switzerland: World Health Organization,
2021
).
This content is only available via PDF.
You do not currently have access to this content.