We report laser desorption/ionization mass spectrometry using a dye-doped nanoporous polymer matrix. The nanoporous polymer matrix was fabricated through a holographic interference patterning technique. The periodically aligned nanopores in the resulting polymer matrix produced a high surface-to-volume ratio that facilitates the homogeneous cocrystallization of the matrix and an analyte (i.e., peptide in this demonstration). To generate nanostructures with further enhanced functionalities, dyes were also incorporated into the photopolymer. We demonstrate that by using the dye-doped nanoporous polymer matrix, we can identify peptides with an enhanced signal from the peptides and decreased noise from the ion fragmentation. These results indicate that the dye-doped nanoporous polymer matrix we use here can be a promising platform for laser desorption/ionization mass spectrometry.
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e-mail: kshsiao@ncnu.edu.tw
e-mail: yzz113@gmail.com
e-mail: hsb6@psu.edu
e-mail: btk5051@psu.edu
e-mail: wyan.nc@gmail.com
e-mail: pamela.lloyd@wpafb.af.mil
e-mail: timothy.bunning@wpafb.af.mil
e-mail: anc@buffalo.edu
e-mail: junhuang@psu.edu
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November 2010
Research Papers
Holographically Fabricated Dye-Doped Nanoporous Polymers as Matrix for Laser Desorption/Ionization Mass Spectrometry
Vincent K. S. Hsiao,
Vincent K. S. Hsiao
Department of Applied Materials and Optoelectronic Engineering,
e-mail: kshsiao@ncnu.edu.tw
National Chi Nan University
, Nantou, 54561 Taiwan; Department of Engineering Science and Mechanics, The Pennsylvania State University
, University Park, PA 16802
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Yue Bing Zheng,
Yue Bing Zheng
Department of Engineering Science and Mechanics,
e-mail: yzz113@gmail.com
The Pennsylvania State University
, University Park, PA 16802
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Heike Betz,
Heike Betz
The Huck Institutes of the Life Science,
e-mail: hsb6@psu.edu
The Pennsylvania State University
, University Park, PA 16802
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Brian Kiraly,
Brian Kiraly
Department of Engineering Science and Mechanics,
e-mail: btk5051@psu.edu
The Pennsylvania State University
, University Park, PA 16802
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Wei Yan,
Wei Yan
Department of Engineering Science and Mechanics,
e-mail: wyan.nc@gmail.com
The Pennsylvania State University
, University Park, PA 16802
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Pamela F. Lloyd,
Pamela F. Lloyd
Materials and Manufacturing Directorate, Air Force Research Laboratory,
e-mail: pamela.lloyd@wpafb.af.mil
Wright-Patterson Air Force Base
, Dayton, OH 45433
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Timothy J. Bunning,
Timothy J. Bunning
Materials and Manufacturing Directorate, Air Force Research Laboratory,
e-mail: timothy.bunning@wpafb.af.mil
Wright-Patterson Air Force Base
, Dayton, OH 45433
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Alexander N. Cartwright,
Alexander N. Cartwright
Institute for Lasers, Photonics and Biophotonics,
e-mail: anc@buffalo.edu
University at Buffalo, The State University of New York
, Buffalo, NY 14260-3000
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Tony Jun Huang
Tony Jun Huang
Department of Engineering Science and Mechanics,
e-mail: junhuang@psu.edu
The Pennsylvania State University
, University Park, PA 16802
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Vincent K. S. Hsiao
Department of Applied Materials and Optoelectronic Engineering,
National Chi Nan University
, Nantou, 54561 Taiwan; Department of Engineering Science and Mechanics, The Pennsylvania State University
, University Park, PA 16802e-mail: kshsiao@ncnu.edu.tw
Yue Bing Zheng
Department of Engineering Science and Mechanics,
The Pennsylvania State University
, University Park, PA 16802e-mail: yzz113@gmail.com
Heike Betz
The Huck Institutes of the Life Science,
The Pennsylvania State University
, University Park, PA 16802e-mail: hsb6@psu.edu
Brian Kiraly
Department of Engineering Science and Mechanics,
The Pennsylvania State University
, University Park, PA 16802e-mail: btk5051@psu.edu
Wei Yan
Department of Engineering Science and Mechanics,
The Pennsylvania State University
, University Park, PA 16802e-mail: wyan.nc@gmail.com
Pamela F. Lloyd
Materials and Manufacturing Directorate, Air Force Research Laboratory,
Wright-Patterson Air Force Base
, Dayton, OH 45433e-mail: pamela.lloyd@wpafb.af.mil
Timothy J. Bunning
Materials and Manufacturing Directorate, Air Force Research Laboratory,
Wright-Patterson Air Force Base
, Dayton, OH 45433e-mail: timothy.bunning@wpafb.af.mil
Alexander N. Cartwright
Institute for Lasers, Photonics and Biophotonics,
University at Buffalo, The State University of New York
, Buffalo, NY 14260-3000e-mail: anc@buffalo.edu
Tony Jun Huang
Department of Engineering Science and Mechanics,
The Pennsylvania State University
, University Park, PA 16802e-mail: junhuang@psu.edu
J. Nanotechnol. Eng. Med. Nov 2010, 1(4): 041011 (5 pages)
Published Online: October 29, 2010
Article history
Received:
September 8, 2010
Revised:
September 21, 2010
Online:
October 29, 2010
Published:
October 29, 2010
Citation
Hsiao, V. K. S., Zheng, Y. B., Betz, H., Kiraly, B., Yan, W., Lloyd, P. F., Bunning, T. J., Cartwright, A. N., and Huang, T. J. (October 29, 2010). "Holographically Fabricated Dye-Doped Nanoporous Polymers as Matrix for Laser Desorption/Ionization Mass Spectrometry." ASME. J. Nanotechnol. Eng. Med. November 2010; 1(4): 041011. https://doi.org/10.1115/1.4002610
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