Process-induced damage to cells is of significant importance and must be mitigated for safe and reproducible cell direct writing. The objective of this study is to investigate the cell membrane stability under the external normal pressure. This investigation is performed by studying the dipalmitoylphosphatidylcholine bilayer behavior under different normal pressures using molecular dynamics. As the normal pressure increases, the force necessary to rupture the bilayer structure decreases, which indicates cell membrane instability under high normal pressure. This phenomenon can also be explained by the change of free energy difference before and after rupture under different normal pressures. The effect of the pulling speed on the rupture force is also investigated, showing that the rupture force increases almost linearly with the pulling speed.

References

1.
Barron
,
J. A.
,
Wu
,
P.
,
Ladouceur
,
H. D.
, and
Ringeisen
,
B. R.
, 2004, “
Biological Laser Printing: A Novel Technique for Creating Heterogeneous 3-dimensional Cell Patterns
,”
Biomed. Microdevices
,
6
(
2
), pp.
139
147
.
2.
Lin
,
Y.
,
Huang
,
Y.
,
Wang
,
G.
,
Tzeng
,
J.
, and
Chrisey
,
D. B.
, 2009, “
Effect of Laser Fluence on Yeast Cell Viability in Laser-Assisted Cell Transfer
,”
J. Appl. Phys.
,
106
, p.
043106
.
3.
Lin
,
Y.
,
Huang
,
G.
,
Huang
,
Y.
,
Tzeng
,
T. J.
, and
Chrisey
,
D. B.
, 2010, “
Effect of Laser Fluence in Laser-Assisted Direct Writing of Human Colon Cancer Cell
,”
Rapid Prototyping J.
,
16
, pp.
202
208
.
4.
Wang
,
W.
,
Huang
,
Y.
, and
Chrisey
,
D. B.
, 2007, “
Numerical Study of Cell Droplet and Hydrogel Coating Impact Process in Cell Direct Writing
,”
Trans. NAMRI/SME
,
35
, pp.
217
224
.
5.
Wang
,
W.
,
Huang
,
Y.
,
Grujicic
,
M.
, and
Chrisey
,
D. B.
, 2008, “
Study of Impact-Induced Mechanical Effects in Cell Direct Writing Using Smooth Particle Hydrodynamic Method
,”
ASME J. Manuf. Sci. Eng.
,
130
, p.
021012
.
6.
Wang
,
W.
,
Li
,
G.
, and
Huang
,
Y.
, 2009, “
Modeling of Bubble Expansion-Induced Cell Mechanical Profile in Laser-Assisted Cell Direct Writing
,”
ASME J. Manuf. Sci. Eng.
,
131
, p.
051013
.
7.
Lin
,
Y.
,
Huang
,
Y.
, and
Chrisey
,
D.
, 2011, “
Metallic Foil-Assisted Laser Cell Printing
,”
ASME J. Biomech. Eng.
,
133
, p.
025001
.
8.
Singer
,
S. J.
, and
Nicolson
,
G. L.
, 1972, “
The Fluid Mosaic Model of the Structure of Cell Membranes
,”
Science
,
175
, pp.
720
731
.
9.
Simons
,
K.
, and
Ikonen
,
E.
, 1997, “
Functional Rafts in Cell Membranes
,”
Nature (London)
,
387
, pp.
569
572
.
10.
Leontiadou
,
H.
,
Mark
,
A. E.
, and
Marrink
,
S. J.
, 2004, “
Molecular Dynamics Simulations of Hydrophilic Pores in Lipid Bilayers
,”
Biophys. J.
,
86
, pp.
2156
2164
.
11.
van der Spoel
,
D.
,
Lindahl
,
E.
,
Hess
,
B.
,
van Buuren
,
A. R.
,
Apol
,
E.
,
Meulenhoff
,
P. J.
,
Tieleman
,
D. P.
,
Sijbers
,
A. L. T. M.
,
Feenstra
,
K. A.
,
van Drunen
,
R.
, and
Berendsen
,
H. J. C.
, 2005, GROMACS User Manual Version 3.3, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
12.
van Gunsteren
,
W. F.
,
Billeter
,
S. R.
,
Eising
,
A. A.
,
Hunenberger
,
P. H.
,
Kruger
,
P.
,
Mark
,
A. E.
,
Scott
,
W. R. P.
, and
Tironi
,
I. G.
, 1996,
Biomolecular Simulation: The GROMOS96 Manual and User Guide
,
vdf Hochschulverlag, ETH, Zurich
,
Switzerland
.
13.
Tieleman
,
D. P.
, and
Berendsen
,
H. J. C.
, 1996, “
Molecular Dynamics Simulations of A Fully Hydrated Dipalmitoyl phosphatidylcholine Bilayer With Different Macroscopic Boundary Conditions and Parameters
,”
J. Chem. Phys.
,
105
, pp.
4871
4880
.
14.
Patel
,
R. Y.
, and
Balaji
,
P. V.
, 2005, “
Effect of the Choice of the Pressure Coupling Method on the Spontaneous Aggregation of DPPC Molecules
,”
J. Phys. Chem. B
,
109
, pp.
14667
14674
.
15.
Grubmüller
,
H.
,
Heymann
,
B.
, and
Tavan
,
P.
, 1996, “
Ligand Binding: Molecular Mechanics Calculation of the Streptavidin-Biotin Rupture Force
,”
Science
,
271
, pp.
997
999
.
16.
Yin
,
J.
, and
Zhao
,
Y. P.
, 2007, “
Molecular Dynamics Simulation of Adhesion Forces in A Dipalmitoylphosphatidylcholine Membrane
,”
Surf. Rev. Lett.
,
14
, pp.
671
675
.
17.
Cleary
,
S. F.
, 1977, “
Laser Pulses and the Generation of Acoustic Transients in Biological Material
,”
Laser Applications in Medicine and Surgery
,
M. L.
Wolbarst
, ed.,
Plenum
,
New York
, Vol.
3
, pp.
175
219
.
18.
Rossignol
,
N.
,
Moan
,
R.
,
Jaouen
,
P.
,
Robert
,
J.
, and
Quemeneur
,
F.
, 1999, “
Continuous High-Pressure Disruption of Marine Diatom Haslea Ostrearia. Assessment by Laser Diffraction Particle Sizer
,”
Biotechnol. Tech.
,
13
, pp.
909
913
.
19.
Gonzalez
,
M. E.
,
Anthon
,
G. E.
, and
Barrett
,
D. M.
, 2010, “
Onion Cells After High Pressure and Thermal Processing Comparison of Membrane Integrity Changes Using Different Analytical Methods and Impact on Tissue Texture
,”
J. Food Sci.
,
75
, pp.
E426
E432
.
20.
Weiner
,
J. H.
, 1983,
Statistical Mechanics of Elasticity
,
John Wiley & Sons
,
New York
.
21.
Litster
,
J. D.
, 1975, “
Stability of Lipid Bilayers and Red Blood Cell Membranes
,”
Phys. Lett. A
,
53
, pp.
193
194
.
22.
Rawicz
,
W.
,
Olbrich
,
K. C.
,
Mclntosh
,
T.
,
Needham
,
D.
, and
Evans
,
E.
, 2000, “
Effect of Chain Length and Unsaturation on Elasticity of Lipid Bilayers
,”
Biophys. J.
,
79
, pp.
328
339
.
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