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Keywords: cryopreservation
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Journal Articles
Publisher: ASME
Article Type: Research-Article
J Biomech Eng. July 2020, 142(7): 071006.
Paper No: BIO-19-1207
Published Online: March 4, 2020
...-adaptive (SA) modification for the purpose of optimizing the compositions of dimethylsufloxide (DMSO)-free cryoprotectants. Post-thaw recovery of Jurkat cells cryopreserved with two DMSO-free cryoprotectants at a cooling rate of 1 °C/min displayed a nonlinear, four-dimensional structure with multiple...
Journal Articles
Publisher: ASME
Article Type: Research-Article
J Biomech Eng. August 2019, 141(8): 081011.
Paper No: BIO-19-1106
Published Online: May 13, 2019
... matter. The field of modern cryopreservation, or preservation of biological matter at subfreezing temperatures, emerged from the 1948 discovery that certain chemical additives such as glycerol, known as cryoprotectants, can protect cells from freeze-related damage by depressing the freezing point...
Includes: Supplementary data
Journal Articles
Publisher: ASME
Article Type: Research-Article
J Biomech Eng. September 2013, 135(9): 091001.
Paper No: BIO-12-1240
Published Online: July 10, 2013
... cryopreservation, ice forms in the extracellular space resulting in freezing-induced deformation of the tissue, which can be detrimental to the extracellular matrix (ECM) microstructure. Meanwhile, cells dehydrate through an osmotically driven process as the intracellular water is transported to the extracellular...
Journal Articles
Publisher: ASME
Article Type: Research Papers
J Biomech Eng. June 2012, 134(6): 061005.
Published Online: June 13, 2012
...Jamie Wright; Bumsoo Han; Cheng-Jen Chuong Cryopreservation of engineered tissue (ET) has achieved limited success due to limited understanding of freezing-induced biophysical phenomena in ETs, especially fluid-matrix interaction within ETs. To further our understanding of the freezing-induced...
Journal Articles
Publisher: ASME
Article Type: Research Papers
J Biomech Eng. February 2011, 133(2): 021007.
Published Online: January 31, 2011
...Xiaoming Zhou; Zhong Liu; Zhiquan Shu; Weiping Ding; Pingan Du; JaeHyun Chung; Carolyn Liu; Shelly Heimfeld; Dayong Gao In most cryopreservation applications, the final concentrations of cryoprotective agents (CPAs) must be reduced to biocompatible levels. However, traditional methods for removing...
Journal Articles
Publisher: ASME
Article Type: Research Papers
J Biomech Eng. March 2010, 132(3): 031003.
Published Online: February 4, 2010
...Ka Yaw Teo; J. Craig Dutton; Bumsoo Han In order to cryopreserve functional engineered tissues (ETs), the microstructure of the extracellular matrix (ECM) should be maintained, as well as the cellular viability since the functionality is closely related to the ECM microstructure. Since the post...
Journal Articles
Journal Articles
Publisher: ASME
Article Type: Technical Papers
J Biomech Eng. October 2007, 129(5): 688–694.
Published Online: February 14, 2007
... + 25 ° C to + 4 ° C before being frozen. These observations suggest that these variables ought to be considered in the derivation of methods to cryopreserve ovarian tissues. The total difference between q initial and q final is denoted as Δ q DSC and the temperature...
Journal Articles
Publisher: ASME
Article Type: Article
J Biomech Eng. April 2005, 127(2): 295–300.
Published Online: December 6, 2004
.... , Olin , T. , Vincente , W. , Troedsson , M. H. T. , Bischof , J. C. , and Roberts , K. P. , 2002 , “ Cryopreservation of Equine Spermatozoa: Optimal Cooling Rates in the Presence and Absence of Cryoprotective Agents ,” Biol. Reprod. 0006-3363 66 , pp. 222 – 231...