Microfabrication has become widely utilized to generate controlled microenvironments that establish chemical concentration gradients for a variety of engineering and life science applications. To establish microfluidic flow, the majority of existing devices rely upon additional facilities, equipment, and excessive reagent supplies, which together limit device portability as well as constrain device usage to individuals trained in technological disciplines. The current work presents our laboratory-developed bridged system, which is a stand-alone device that runs via conventional pipette loading and can operate for several days without need of external machinery or additional reagent volumes. The bridged is a two-layer polydimethylsiloxane microfluidic device that is able to establish controlled chemical concentration gradients over time by relying solely upon differences in reagent densities. Fluorescently labeled Dextran was used to validate the design and operation of the bridged by evaluating experimentally measured transport properties within the microsystem in conjunction with numerical simulations and established mathematical transport models. Results demonstrate how the bridged system was used to generate spatial concentration gradients that resulted in an experimentally measured Dextran diffusivity of .
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e-mail: vazquez@ccny.cuny.edu
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December 2010
Research Papers
A Microfluidic Device to Establish Concentration Gradients Using Reagent Density Differences
Qingjun Kong,
Qingjun Kong
Department of Biomedical Engineering,
The City College of The City University of New York (CCNY)
, Room 403D, Steinman Hall, 160 Convent Avenue, New York, NY 10031
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Richard A. Able, Jr.,
Richard A. Able, Jr.
Department of Biomedical Engineering,
The City College of The City University of New York (CCNY)
, Room 403D, Steinman Hall, 160 Convent Avenue, New York, NY 10031
Search for other works by this author on:
Veronica Dudu,
Veronica Dudu
Department of Biomedical Engineering,
The City College of The City University of New York (CCNY)
, Room 403D, Steinman Hall, 160 Convent Avenue, New York, NY 10031
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Maribel Vazquez
Maribel Vazquez
Department of Biomedical Engineering,
e-mail: vazquez@ccny.cuny.edu
The City College of The City University of New York (CCNY)
, Room 403D, Steinman Hall, 160 Convent Avenue, New York, NY 10031
Search for other works by this author on:
Qingjun Kong
Department of Biomedical Engineering,
The City College of The City University of New York (CCNY)
, Room 403D, Steinman Hall, 160 Convent Avenue, New York, NY 10031
Richard A. Able, Jr.
Department of Biomedical Engineering,
The City College of The City University of New York (CCNY)
, Room 403D, Steinman Hall, 160 Convent Avenue, New York, NY 10031
Veronica Dudu
Department of Biomedical Engineering,
The City College of The City University of New York (CCNY)
, Room 403D, Steinman Hall, 160 Convent Avenue, New York, NY 10031
Maribel Vazquez
Department of Biomedical Engineering,
The City College of The City University of New York (CCNY)
, Room 403D, Steinman Hall, 160 Convent Avenue, New York, NY 10031e-mail: vazquez@ccny.cuny.edu
J Biomech Eng. Dec 2010, 132(12): 121012 (9 pages)
Published Online: November 16, 2010
Article history
Received:
March 25, 2010
Revised:
September 17, 2010
Posted:
October 15, 2010
Published:
November 16, 2010
Online:
November 16, 2010
Citation
Kong, Q., Able, R. A., Jr., Dudu, V., and Vazquez, M. (November 16, 2010). "A Microfluidic Device to Establish Concentration Gradients Using Reagent Density Differences." ASME. J Biomech Eng. December 2010; 132(12): 121012. https://doi.org/10.1115/1.4002797
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