Morpholinos (MOs) are synthetic nucleic acids analogues with a non-charged backbone of morpholine rings. To enhance the MO-DNA hybridization assay speed, we propose the integration of a MO microarray with an ion concentration polarization (ICP) based microfluidic concentrator. The ICP concentrator collects target biomolecules from a ∼μL fluidic DNA sample and concentrates them electrokinetically into a ∼nL plug located in the vicinity of the MO probes. ICP preconcentration not only reduces the analyte diffusion length but also increases the binding reaction rate, and as a result, ICP-enhanced MO microarrays allow much faster hybridization than standard diffusion-limited MO microarrays.
- Heat Transfer Division
Accelerating the Mass Transport of DNA Biomolecules Onto DNA Microarray for Enhanced Detection by Electrokinetic Concentration in a Microfluidic Chip
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Martins, D, Wei, X, Levicky, R, & Song, Y. "Accelerating the Mass Transport of DNA Biomolecules Onto DNA Microarray for Enhanced Detection by Electrokinetic Concentration in a Microfluidic Chip." Proceedings of the ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer. Volume 1: Micro/Nanofluidics and Lab-on-a-Chip; Nanofluids; Micro/Nanoscale Interfacial Transport Phenomena; Micro/Nanoscale Boiling and Condensation Heat Transfer; Micro/Nanoscale Thermal Radiation; Micro/Nanoscale Energy Devices and Systems. Biopolis, Singapore. January 4–6, 2016. V001T01A007. ASME. https://doi.org/10.1115/MNHMT2016-6562
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