The constraint-based design of flexure mechanisms requires a qualitative and quantitative understanding of the constraint characteristics of flexure elements that serve as constraints. This paper presents the constraint characterization of a uniform and symmetric cross-section, slender, spatial beam—a basic flexure element commonly used in three-dimensional flexure mechanisms. The constraint characteristics of interest, namely stiffness and error motions, are determined from the nonlinear load–displacement relations at the beam end. Appropriate assumptions are made while formulating the strain and strain energy expressions for the spatial beam to retain relevant geometric nonlinearities. Using the principle of virtual work, nonlinear beam governing equations are derived and subsequently solved for general end loads. The resulting nonlinear load–displacement relations capture the constraint characteristics of the spatial beam in a compact, closed-form, and parametric manner. This constraint model is shown to be accurate using nonlinear finite element analysis, within a load and displacement range of practical interest. The utility of this model lies in the physical and analytical insight that it offers into the constraint behavior of a spatial beam flexure, its use in design and optimization of 3D flexure mechanism geometries, and its elucidation of fundamental performance tradeoffs in flexure mechanism design.